dating female london myspace com site uk - Amidating
Different amidation events on chromogranin A, and on peptides processed from proopiomelanocortin, manifest similar striking sensitivity to hypoxia in a range of neuroendocrine cells, being progressively inhibited from mild (7% O2) to severe (1% O2) hypoxia.
In developing Drosophila melanogaster larvae, FMRF amidation in thoracic ventral (Tv) neurons is strikingly suppressed by hypoxia.
Peptidyl glycine alpha-amidating monooxygenase (PAM) catalyzes a two-step reaction resulting in the C-terminal amidation of peptides, a process important for their stability and biological activity.
Interestingly, in Drosophila, the PHM and PAL enzyme are not fused.
The Drosophila genome predicts expression of one monofunctional PHM gene and two monofunctional PAL genes [PMID: 15198673].
These catalytic domains work sequentially to catalyze neuroendocrine peptides to active alpha-amidated products.
Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene, but some of their full-length sequences are not yet known., this process relies on a copper cofactor.
Results: Modification-specific antibodies reveal that peptide substrate amidation is strikingly sensitive to the exposure of cells to moderate hypoxia.